The importance of non-coding RNAs (ncRNAs) in controlling gene expression is becoming increasingly evident. However, except for some well characterized examples, such as miRNAs, Xist and Air, the function of mostnon-coding transcripts is still to be determined. Moreover, while small regulatory RNAs can be relatively easily classified on the basis of their length, secondary structure, and biochemical pathway, the classification of long “mRNA-like” ncRNAs has been problematic.Here we identify a large class of non-coding transcripts that originate within the 3’UTR of at least one third of all genes in the mouse genome. We have several lines of evidence from genome-wide bioinformatic analyses (EST coverage, CAGE data, chromatin state maps of active promoters) and from invitro studies (strand-specific RT-PCR, 5’RACE, Northern blot) showing that these 3’UTR-associated ncRNAs (uaRNAs) can be either linked or transcribed separately to the upstream protein-coding sequences. In addition, expressionprofiles obtained by custom-designed microarrays on three differentdevelopmental systems (myoblast differentiation, male gonadal ridgeformation, embryonic stem cell differentiation) showed that uaRNA expressionis highly regulated and tissue-specific, and might be either concordant ordiscordant with respect to the upstream coding region depending on the celltype and on the developmental stage. This observation is confirmed by in-situhybridization experiments, which evidenced that uaRNA and the associatedcoding transcript might have different subcellular locations.Our results highlight a further level of complexity at 3’UTRs, suggesting thepresence of new regulatory mechanisms that control gene expression duringembryonic development. Our data have also important implications for thedesign of in-situ hybridization and microarray probes as well as for theinterpretation of gene expression data

A new class of non-coding RNAs associated with 3’ untranslated regions of mRNAs

G. Soldà;
2008-01-01

Abstract

The importance of non-coding RNAs (ncRNAs) in controlling gene expression is becoming increasingly evident. However, except for some well characterized examples, such as miRNAs, Xist and Air, the function of mostnon-coding transcripts is still to be determined. Moreover, while small regulatory RNAs can be relatively easily classified on the basis of their length, secondary structure, and biochemical pathway, the classification of long “mRNA-like” ncRNAs has been problematic.Here we identify a large class of non-coding transcripts that originate within the 3’UTR of at least one third of all genes in the mouse genome. We have several lines of evidence from genome-wide bioinformatic analyses (EST coverage, CAGE data, chromatin state maps of active promoters) and from invitro studies (strand-specific RT-PCR, 5’RACE, Northern blot) showing that these 3’UTR-associated ncRNAs (uaRNAs) can be either linked or transcribed separately to the upstream protein-coding sequences. In addition, expressionprofiles obtained by custom-designed microarrays on three differentdevelopmental systems (myoblast differentiation, male gonadal ridgeformation, embryonic stem cell differentiation) showed that uaRNA expressionis highly regulated and tissue-specific, and might be either concordant ordiscordant with respect to the upstream coding region depending on the celltype and on the developmental stage. This observation is confirmed by in-situhybridization experiments, which evidenced that uaRNA and the associatedcoding transcript might have different subcellular locations.Our results highlight a further level of complexity at 3’UTRs, suggesting thepresence of new regulatory mechanisms that control gene expression duringembryonic development. Our data have also important implications for thedesign of in-situ hybridization and microarray probes as well as for theinterpretation of gene expression data
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11699/11473
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