Clonal CD8+ TCR-Vbeta expanded populations with effector memory phenotype in Churg Strauss syndrome

Giuseppe, Guida; Antonella, Vallario; Stefania, Stella; Monica, Boita; Paola, Circosta; Sara, Mariani; Giuseppina, Prato; Heffler, Enrico Marco; Roberta, Bergia; Antonino, Sottile; Giovanni, Rolla; Alessandro, Cignetti

doi:10.1016/j.clim.2008.03.505

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Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vbeta expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansions in 8 CSS patients, 10 asthmatics and 42 healthy subjects. In all CSS patients one or two Vbeta families were expanded among CD8+ cells, with an effector memory phenotype apt to populate tissues and inflammatory sites. Heteroduplex PCR showed the presence of one or more clonal TCR rearrangements, which reveals monoclonal or oligoclonal T cells subpopulations. After purification with a Vbeta specific monoclonal antibody, each CD8+/Vbeta+ expanded family showed a single TCR rearrangement, clearly suggestive of monoclonality. All CD8+ expansions were detectable throughout the disease course. TCR-Vbeta expanded or deleted populations were not observed in asthmatic patients. Clonal CD8+/Vbeta+ T cell expansions might be useful as a disease marker.

Titolo:	Clonal CD8+ TCR-Vbeta expanded populations with effector memory phenotype in Churg Strauss syndrome
Autori:	Giuseppe Guida Antonella Vallario Stefania Stella Monica Boita Paola Circosta Sara Mariani Giuseppina Prato HEFFLER, Enrico Marco Roberta Bergia Antonino Sottile Giovanni Rolla Alessandro Cignetti mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2008
Rivista:	CLINICAL IMMUNOLOGY
Abstract:	Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vbeta expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansions in 8 CSS patients, 10 asthmatics and 42 healthy subjects. In all CSS patients one or two Vbeta families were expanded among CD8+ cells, with an effector memory phenotype apt to populate tissues and inflammatory sites. Heteroduplex PCR showed the presence of one or more clonal TCR rearrangements, which reveals monoclonal or oligoclonal T cells subpopulations. After purification with a Vbeta specific monoclonal antibody, each CD8+/Vbeta+ expanded family showed a single TCR rearrangement, clearly suggestive of monoclonality. All CD8+ expansions were detectable throughout the disease course. TCR-Vbeta expanded or deleted populations were not observed in asthmatic patients. Clonal CD8+/Vbeta+ T cell expansions might be useful as a disease marker.
Handle:	http://hdl.handle.net/11699/13239
Appare nelle tipologie:	1.1 Articolo in rivista

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