Background. Bile juice plays a major role in duodenogastroesophageal reflux (DGERD). Several devices to directly measure the bileconcentration have been proposed. We aimed to ex-vivo evaluate the bile concentration by narrow band imaging (NBI). Method.From six surgical cholecystectomies, the content of the gallbladders was aspirated and the total biliary acid (TBA) concentrationwas evaluated. 2 mL was employed for serial twofold dilutions. Each dilution was scoped. Images on white light (WL) and NBI werecaptured and grouped accordingly to NBI-appearance and TBA-concentration. Results. Nondiluted bile had a TBA-concentrationof 61965 ± 32989 mol/L. Final dilution (1 : 4096) had 1.16 mol/L. NBI and correspondent WL images were grouped into sevengroups, and an NBI/Bile scale was created. Conclusion. The scale showed that not only NBI scale but also white light scale could beuseful to predict the bile concentration.This initial study shows that NBI has a potential role in the detection of DGERD and furtherinvestigation is warranted to distinguish the presence and the concentration of bile, especially at very low TBA concentrations.
Endoscopic ex vivo evaluation of bile concentrations by narrow band imaging: a pilot study
MASELLI, ROBERTA;
2015-01-01
Abstract
Background. Bile juice plays a major role in duodenogastroesophageal reflux (DGERD). Several devices to directly measure the bileconcentration have been proposed. We aimed to ex-vivo evaluate the bile concentration by narrow band imaging (NBI). Method.From six surgical cholecystectomies, the content of the gallbladders was aspirated and the total biliary acid (TBA) concentrationwas evaluated. 2 mL was employed for serial twofold dilutions. Each dilution was scoped. Images on white light (WL) and NBI werecaptured and grouped accordingly to NBI-appearance and TBA-concentration. Results. Nondiluted bile had a TBA-concentrationof 61965 ± 32989 mol/L. Final dilution (1 : 4096) had 1.16 mol/L. NBI and correspondent WL images were grouped into sevengroups, and an NBI/Bile scale was created. Conclusion. The scale showed that not only NBI scale but also white light scale could beuseful to predict the bile concentration.This initial study shows that NBI has a potential role in the detection of DGERD and furtherinvestigation is warranted to distinguish the presence and the concentration of bile, especially at very low TBA concentrations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
