A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C-18 (4.6x150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1),and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 mug/ml (r(2)greater than or equal to0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 mug/ml. Using this method IDN 5390 pharmacokinetics was determined in mice. (C) 2002 Elsevier Science B.V. All rights reserved.
High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma
D'Incalci M;
2002-01-01
Abstract
A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C-18 (4.6x150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1),and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 mug/ml (r(2)greater than or equal to0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 mug/ml. Using this method IDN 5390 pharmacokinetics was determined in mice. (C) 2002 Elsevier Science B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
