Antimitochondrial antibodies (AMAs) are the hallmark of primary biliary cholangitis (PBC) but can be identified also in patients with connective tissue disease, namely, systemic sclerosis (SSc). Protein immunoprecipitation (IP) and IP-Western blot (WB) can be used to confirm AMA positivity directed at the pyruvate dehydrogenase complex (PDC) subunits E1α, E1β, E2/E3, and E3BP in patients showing a cytoplasmic reticular pattern at indirect immunofluorescence when performed in a screening setting before the onset of overt cholestasis in rheumatic patients.

Antigen Reactivity and Clinical Significance of Autoantibodies Directed Against the Pyruvate Dehydrogenase Antigen Complex in Patients With Connective Tissue Disease

Ceribelli, Angela;De Santis, Maria;Selmi, Carlo
2022

Abstract

Antimitochondrial antibodies (AMAs) are the hallmark of primary biliary cholangitis (PBC) but can be identified also in patients with connective tissue disease, namely, systemic sclerosis (SSc). Protein immunoprecipitation (IP) and IP-Western blot (WB) can be used to confirm AMA positivity directed at the pyruvate dehydrogenase complex (PDC) subunits E1α, E1β, E2/E3, and E3BP in patients showing a cytoplasmic reticular pattern at indirect immunofluorescence when performed in a screening setting before the onset of overt cholestasis in rheumatic patients.
IP-Western blot
antimitochondrial antibodies (AMAs)
primary biliary cholangitis (PBC)
protein immunoprecipitation
pyruvate dehydrogenase complex (PDC)
Autoantibodies
Humans
Mitochondria
Oxidoreductases
Pyruvate Dehydrogenase Complex
Pyruvates
Liver Cirrhosis, Biliary
Scleroderma, Systemic
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11699/68471
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