Myositis specific antibodies (MSA) represent not only important diagnostic tools for idiopathic inflammatory myopathies (IIM), but also help to stratify patients into subsets with particular clinical features, treatment responses, and disease outcome. Consequently, standardization of MSA is of high importance. Although many laboratories rely on protein immunoprecipitation (IP) for the detection of MSA, IP standardization is challenging and therefore reliable alternatives are mandatory. Recently, we identified significant variation between IP and line immunoassay (LIA) for the detection of MSA and myositis associated antibodies. In this study we aimed to compare the results from our previous study to the results obtained with a novel fully automated particle-based technology for the detection of MSA and MAA.

Evaluation of a novel particle-based assay for detection of autoantibodies in idiopathic inflammatory myopathies

Ceribelli, A;
2019

Abstract

Myositis specific antibodies (MSA) represent not only important diagnostic tools for idiopathic inflammatory myopathies (IIM), but also help to stratify patients into subsets with particular clinical features, treatment responses, and disease outcome. Consequently, standardization of MSA is of high importance. Although many laboratories rely on protein immunoprecipitation (IP) for the detection of MSA, IP standardization is challenging and therefore reliable alternatives are mandatory. Recently, we identified significant variation between IP and line immunoassay (LIA) for the detection of MSA and myositis associated antibodies. In this study we aimed to compare the results from our previous study to the results obtained with a novel fully automated particle-based technology for the detection of MSA and MAA.
Idiopathic inflammatory myopathies
Immunoprecipitation
Myositis
Myositis specific antibodies
Solid phase assays
Standardization
Autoantibodies
Automation, Laboratory
Biomarkers
Humans
Immunoprecipitation
Myositis
Predictive Value of Tests
Reproducibility of Results
Immunoassay
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11699/68486
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