Autoantibodies to the Th/To antigen have been described in systemic sclerosis (SSc) and several proteins of the macromolecular Th/To complex have been reported to react with anti-Th/To antibodies. However, anti-Th/To has not been clinically utilized due to unavailability of commercial tests. The objective of the present study is to evaluate the newly developed ELISA and chemiluminescent immunoassay (CLIA) to measure autoantibodies to Rpp25 (a component of the Th/To complex) using immunoprecipitation (IP) as the reference method.

Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay

Ceribelli, Angela;
2013

Abstract

Autoantibodies to the Th/To antigen have been described in systemic sclerosis (SSc) and several proteins of the macromolecular Th/To complex have been reported to react with anti-Th/To antibodies. However, anti-Th/To has not been clinically utilized due to unavailability of commercial tests. The objective of the present study is to evaluate the newly developed ELISA and chemiluminescent immunoassay (CLIA) to measure autoantibodies to Rpp25 (a component of the Th/To complex) using immunoprecipitation (IP) as the reference method.
Area Under Curve
Autoantibodies
Autoantigens
Endoribonucleases
Enzyme-Linked Immunosorbent Assay
Humans
Immunoprecipitation
Luminescent Measurements
Multienzyme Complexes
ROC Curve
Recombinant Proteins
Ribonuclease P
Scleroderma, Systemic
Sensitivity and Specificity
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11699/68507
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