Classic anti-nucleolar antibodies anti-Th/To and U3 ribonucleoprotein (-U3RNP) can help in the diagnosis, prediction of organ involvement and prognosis in systemic sclerosis (SSc); however, no validated commercial assay is available. We aimed at establishing a novel quantitative real time PCR (qPCR) method to detect these antibodies.

A new immunoprecipitation-real time quantitative PCR assay for anti-Th/To and anti-U3RNP antibody detection in systemic sclerosis

Ceribelli, Angela;
2012

Abstract

Classic anti-nucleolar antibodies anti-Th/To and U3 ribonucleoprotein (-U3RNP) can help in the diagnosis, prediction of organ involvement and prognosis in systemic sclerosis (SSc); however, no validated commercial assay is available. We aimed at establishing a novel quantitative real time PCR (qPCR) method to detect these antibodies.
Antibodies, Antinuclear
Autoantigens
Cell Nucleolus
Humans
Immunoprecipitation
K562 Cells
Real-Time Polymerase Chain Reaction
Ribonucleoproteins
Scleroderma, Systemic
Sensitivity and Specificity
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11699/68513
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