Objective: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. © 2010 American Heart Association, Inc.

Role of delta-like-4/notch in the formation and wiring of the lymphatic network in zebrafish

Mazzone M.;
2010-01-01

Abstract

Objective: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. © 2010 American Heart Association, Inc.
2010
gene silencing
lymphangiogenesis
notch signaling
vascular biology
zebrafish
Animals
Animals
Genetically Modified
Biomarkers
COS Cells
Cell Movement
Cell Proliferation
Chlorocebus aethiops
Coculture Techniques
Embryo
Nonmammalian
Endothelial Cells
Gene Expression Regulation
Developmental
Gene Knockdown Techniques
Gene Silencing
Humans
Intracellular Signaling Peptides and Proteins
Luminescent Proteins
Lymphatic System
Membrane Proteins
RNA
Messenger
Receptors
Notch
Thoracic Duct
Zebrafish
Zebrafish Proteins
Lymphangiogenesis
Signal Transduction
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11699/83068
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