Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain

Luchetti, A; Milani, D; Ruffini, F; Galli, R; Falini, A; Quattrini, A; Scotti, G; Comi, G; Martino, G; Furlan, R; Politi, L

doi:10.2310/7290.2011.00032

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We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T-2* relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220(+) cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3(+) infiltrates in a murine model of multiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle.

Titolo:	Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain
Autori:	Luchetti A Milani D Ruffini F Galli R Falini A Quattrini A Scotti G Comi G Martino G Furlan R POLITI, Letterio Salvatore mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2012
Rivista:	MOLECULAR IMAGING
Handle:	http://hdl.handle.net/11699/8396
Appare nelle tipologie:	1.1 Articolo in rivista

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