Objective: The aim of the study was to investigate the use of a novel hyaluronic acid/polycaprolactonematerial formeniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of theimplant with expanded autologous chondrocytes. Two different surgical implantation techniques in a sheepmodel were evaluated. Methods: Twenty-four skeletally mature sheep were treated with total medial meniscusreplacements, while two meniscectomies served as empty controls. The animals were divided into twogroups: cell-free scaffold and scaffold seeded with autologous chondrocytes. Two different surgical techniqueswere compared: in 12 animals, the implantwas sutured to the capsule and to themeniscal ligament; inthe other 12 animals, also a transtibial fixation of the horns was used. The animals were euthanized after 4months. The specimens were assessed by gross inspection and histology. Results: All implants showed excellentcapsular ingrowth at the periphery.Macroscopically, no difference was observed between cell-seededand cell-free groups. Better implant appearance and integrity was observed in the group without transosseoushorns fixation. Using the latter implantation technique, lower joint degeneration was observed in thecell-seeded group with respect to cell-free implants. The histological analysis indicated cellular infiltrationand vascularization throughout the implanted constructs. Cartilaginous tissue formation was significantlymore frequent in the cell-seeded constructs. Conclusion: The current study supports the potential of a novelHYAFF/polycaprolactone scaffold for total meniscal substitution. Seeding of the scaffolds with autologouschondrocytes provides some benefit in the extent of fibrocartilaginous tissue repair.
|Titolo:||Tissue engineering for total meniscal substitution. Animal study in sheep model|
|Data di pubblicazione:||2008|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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